Two of the more fascinating biological phenomena that have been d- covered in recent years are RNA editing and RNA interference. Each of these processes has been found in a cross-section of biological systems, including mammals, viruses, plants, and a range of model organisms (C.
elegans,Dro- phila, and various lower eukaryotes). RNA editing, which results in an RNA product different from that predicted aby the genome, occurs through a variety of mechanisms. Alterations can occur at either the base level, in which one base is changed to another (substitutional editing/base modification), or via the addition and/or deletion of nucleotides relative to the original template (insertion/deletion editing).
RNA interference (RNAi) involves the specific degradation of targeted mRNAs. Although RNA interference, editing, and modification use different enzymes and mechanisms, the targets of each of these reactions are often specified by RNA molecules.
Indeed, the discovery of guide RNAs (gRNAs) that direct nucleotide insertion and deletion in trypa- some mitochondria set the precedent for subsequent discoveries of the small nuclear RNAs (snoRNAs) that target pseudouridylylation and methylation of stable RNAs and the small double-stranded RNA fragments (siRNAs) that mediate RNAi.
Other small RNAs are known to mediate translational regu- tion during development (small temporal RNAs [stRNAs]) and mRNA stab- ity (microRNAs [miRNAs]), and the recent identification of more than a hundred small "noncoding" RNAs has led to the realization that they may represent only the proverbial "tip of the iceberg.
"...the book delivers detailed experimental protocols that are clearly presented and easy to understand...it delivers practical information..." -ChemBioChemSpis treści:Part I. RNA Interference and Gene Silencing RNA Interference: Historical Overview and Significance Mary K.
Montgomery Delivery of Double-Stranded RNA into Caenorhabditis elegans Dawn Hull and Lisa Timmons Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells Amy A.
Caudy and Gregory J. Hannon Analysis of Gene Function in Trypanosoma brucei Using RNA Interference Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, and Elisabetta Ullu Short Hairpin Activated Gene Silencing in Mammalian Cells Patrick J.
Paddison, Amy A. Caudy, Ravi Sachidanandam, and Gregory J. Hannon Geminivirus Vectors for Transient Gene Silencing in Plants Nooduan Muangsan and Dominique Robertson Posttranscriptional Gene Silencing in Plants Susan Varsha Wesley, Chris Helliwell, Ming-Bo Wang, and Peter Waterhouse Identification of microRNAs and Other Tiny Noncoding RNAs aby cDNA Cloning Victor Ambros and Rosalind C.
Lee Part II. RNA Editing and Modification A Historical Perspective on RNA Editing: How the Peculiar and Bizarre Became Mainstream Donna J. Koslowsky Identification of Substrates for Adenosine Deaminases That Act on RNA Daniel P.
Morse Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coli Gillian M. Ring, Mary A. O'Connell, and Liam P. Keegan Isolation of an mRNA-Binding Protein Involved in C-to-U Editing Carri A.
Gerber, Anne Relich, and Donna M. Driscoll In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities Kenneth Stuart, Reza Salavati, Robert P. Igo, Jr., Nancy Lewis Ernst, Setareh S.
Palazzo, and Bingbing Wang Identification and Characterization of Trypanosome RNA-Editing Complex Components Kenneth Stuart, Aswini K.Panigrahi, and Achim Schnaufer Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro Elaine M.
Byrne Methods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellanii Amanda J. Lohan and Michael W. Gray In Vitro RNA Editing Systems From Higher Plant Chloroplasts Tetsuro Hirose, Tetsuya Miyamoto, Junichi Obokata, and Masahiro Sugiura Studying RNA Editing in Transgenic Chloroplasts of Higher Plants Ralph Bock Detection and Quantification of Modified Nucleotides in RNA Using Thin-Layer Chromatography Henri Grosjean, Gerard Keith, and Louis Droogmans Functional Characterization of 2'-O-Methylation and Pseudouridylation Guide RNAs Tamas Kiss and Beata E.
Jady Experimental RNomics: A Global Approach to Identifying Small Nuclear RNAs and Their Targets in Different Model Organisms Alexander Huttenhofer, Jerome Cavaille, and Jean-Pierre Bachellerie Index